Laboratory of Breeding and Genetic Research on Field Crops

The main research areas are:

– evaluation of soft wheat varieties for the presence of resistance genes to leaf rust using biotechnological and genetic methods;

– field evaluation of wheat and barley varieties for the main diseases in natural and artificial backgrounds;

– study on buckwheat lines using the method of inter simple sequence repeat (ISSR);

– selection of parental pairs for soybean breeding programs using the method of inter simple sequence repeat (ISSR);

– study on the variability of microsatellite loci of F1 soybean hybrids;

– evaluation of immune mechanisms of potato varieties, lines, hybrids and their resistance to phytophagous insects, bacterial and fungal diseases;

– search for resistance genes to pests and viral pathogens, identification of potato viruses by PCR.

Grain crops. The laboratory works on identifying resistance mechanisms of wheat and barley varieties to rust and smut diseases, powdery mildew and Fusarium wilt. Subculturing of Bacillus subtilis was performed. Further research is conducted to obtain true breeding lines of Piricularia oryzae, Beauveria bassiana, Metarhizium anisopliae, Trichoderma fuliginoides, Bacillus subtilis, Bacillus thuringiensis, Fuzarium Martii App apud Panax ginseng.

Buckwheat. Our scientists mastered the methods of DNA extraction and PCR, discovered the distribution pattern of fragments of amplified primers M1 for 8 buckwheat varieties. A research project was set up to evaluate genetic variation of buckwheat lines and varieties, including the lines that were obtained in vitro using a background with heavy metals, and their initial forms by ISSR. Biologically active potential of plants of the genus Reynoutria (Reynoutria Houtt.) and the family Polygonaceae is studied to identify adaptation mechanisms of buckwheat plants to unfavorable environmental conditions.

Soybean. Soybean varieties were studied using 10 primers to select parental pairs. Based on the obtained results, our researchers identified genetic distances and constructed phylogenetic trees for the studied varieties. Our scientists evaluated the hybrid origin of the progeny of three hybrid combinations using 4 pairs of primers, which amplified microsatellite loci, in 2017.

Potato. A research project was set up to evaluate potato varieties for the presence of viruses X and Y, also the potato spindle tuber viroid by PCR analysis. Our scientists conduct studies on resistance mechanisms of potato varieties to the potato ladybug beetle Henosepilachna vigintioctomaculata Motsch, fungal and bacterial diseases. A laboratory experiment was performed on the immune response of potato varieties to Henosepilachna vigintioctomaculata Motsch.