Laboratory of Breeding Genetic Research on Field Crops

Core Research Focus Areas

Genetic and Pathological Evaluation

1. Cereal Crops

   • Identification of leaf rust resistance genes in wheat using molecular markers

   • Field assessment of wheat/barley disease resistance under:
     ✓ Natural infection conditions
     ✓ Controlled pathogen pressure

2. Buckwheat Improvement

   • Genetic characterization using ISSR markers

   • Analysis of primer M1 fragment distribution across 8 varieties

   • Evaluation of heavy metal-adapted in vitro lines

3. Soybean Breeding

   • Parental selection via ISSR profiling (10 primers)

   • Genetic distance analysis and phylogenetic reconstruction

   • Microsatellite locus analysis in F1 hybrids

4. Potato Pathology

   • Virus detection (PVX, PVY, PSTVd) by PCR

   • Resistance mechanisms to:
     • Colorado potato beetle (Henosepilachna vigintioctomaculata)
     • Bacterial/fungal pathogens

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Specialized Research Projects

Microbial Biotechnology

• Isolation and characterization of biocontrol agents:
  Bacillus subtilis, B. thuringiensis, Trichoderma fuliginoides

• Pathogen maintenance:
  Piricularia oryzae, Fusarium Martii, Metarhizium anisopliae

Plant Adaptation Studies

• Screening Reynoutria spp. (Polygonaceae) for:
  ✓ Bioactive compounds
  ✓ Stress-adaptation mechanisms in buckwheat

Advanced Methodologies

• DNA barcoding for pathogen identification

• Microsatellite marker development

• Controlled infection protocols